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gsmtx4 inhibition  (Tocris)


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    Tocris gsmtx4 inhibition
    Fluorescence imaging of calcium oscillation and the effects of LIPUS stimulation on different groups of cells. a After LIPUS stimulation, the Fluo-8-labeled cells exhibited increased intracellular calcium levels at different time points. The red arrows show the two-cell calcium oscillation phenomenon. Scale bars, 50 μm. b Representative intracellular calcium traces of three groups of cells (MC3T3-E1, shRNA-Piezo1, and <t>GsMTx4-treated</t> cells) are shown as the fold increase in Fluo-8 intensity in response to LIPUS stimulation. The experiment was performed for six total minutes, including 1 min of baseline without LIPUS stimulation, 3 min of active stimulation (between the two red lines), and 2 min of regression. Time-lapse sequences were collected every 1.8 s for 6 min. In each field of interest, the fluorescence intensities of 10 cells were quantified using LSM Image Browser software
    Gsmtx4 Inhibition, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsmtx4 inhibition/product/Tocris
    Average 95 stars, based on 76 article reviews
    gsmtx4 inhibition - by Bioz Stars, 2026-04
    95/100 stars

    Images

    1) Product Images from "Piezo1 channel activation in response to mechanobiological acoustic radiation force in osteoblastic cells"

    Article Title: Piezo1 channel activation in response to mechanobiological acoustic radiation force in osteoblastic cells

    Journal: Bone Research

    doi: 10.1038/s41413-020-00124-y

    Fluorescence imaging of calcium oscillation and the effects of LIPUS stimulation on different groups of cells. a After LIPUS stimulation, the Fluo-8-labeled cells exhibited increased intracellular calcium levels at different time points. The red arrows show the two-cell calcium oscillation phenomenon. Scale bars, 50 μm. b Representative intracellular calcium traces of three groups of cells (MC3T3-E1, shRNA-Piezo1, and GsMTx4-treated cells) are shown as the fold increase in Fluo-8 intensity in response to LIPUS stimulation. The experiment was performed for six total minutes, including 1 min of baseline without LIPUS stimulation, 3 min of active stimulation (between the two red lines), and 2 min of regression. Time-lapse sequences were collected every 1.8 s for 6 min. In each field of interest, the fluorescence intensities of 10 cells were quantified using LSM Image Browser software
    Figure Legend Snippet: Fluorescence imaging of calcium oscillation and the effects of LIPUS stimulation on different groups of cells. a After LIPUS stimulation, the Fluo-8-labeled cells exhibited increased intracellular calcium levels at different time points. The red arrows show the two-cell calcium oscillation phenomenon. Scale bars, 50 μm. b Representative intracellular calcium traces of three groups of cells (MC3T3-E1, shRNA-Piezo1, and GsMTx4-treated cells) are shown as the fold increase in Fluo-8 intensity in response to LIPUS stimulation. The experiment was performed for six total minutes, including 1 min of baseline without LIPUS stimulation, 3 min of active stimulation (between the two red lines), and 2 min of regression. Time-lapse sequences were collected every 1.8 s for 6 min. In each field of interest, the fluorescence intensities of 10 cells were quantified using LSM Image Browser software

    Techniques Used: Fluorescence, Imaging, Labeling, shRNA, Software



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    gsmtx4 inhibition  (Tocris)
    95
    Tocris gsmtx4 inhibition
    Fluorescence imaging of calcium oscillation and the effects of LIPUS stimulation on different groups of cells. a After LIPUS stimulation, the Fluo-8-labeled cells exhibited increased intracellular calcium levels at different time points. The red arrows show the two-cell calcium oscillation phenomenon. Scale bars, 50 μm. b Representative intracellular calcium traces of three groups of cells (MC3T3-E1, shRNA-Piezo1, and <t>GsMTx4-treated</t> cells) are shown as the fold increase in Fluo-8 intensity in response to LIPUS stimulation. The experiment was performed for six total minutes, including 1 min of baseline without LIPUS stimulation, 3 min of active stimulation (between the two red lines), and 2 min of regression. Time-lapse sequences were collected every 1.8 s for 6 min. In each field of interest, the fluorescence intensities of 10 cells were quantified using LSM Image Browser software
    Gsmtx4 Inhibition, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsmtx4 inhibition/product/Tocris
    Average 95 stars, based on 1 article reviews
    gsmtx4 inhibition - by Bioz Stars, 2026-04
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Fluorescence imaging of calcium oscillation and the effects of LIPUS stimulation on different groups of cells. a After LIPUS stimulation, the Fluo-8-labeled cells exhibited increased intracellular calcium levels at different time points. The red arrows show the two-cell calcium oscillation phenomenon. Scale bars, 50 μm. b Representative intracellular calcium traces of three groups of cells (MC3T3-E1, shRNA-Piezo1, and GsMTx4-treated cells) are shown as the fold increase in Fluo-8 intensity in response to LIPUS stimulation. The experiment was performed for six total minutes, including 1 min of baseline without LIPUS stimulation, 3 min of active stimulation (between the two red lines), and 2 min of regression. Time-lapse sequences were collected every 1.8 s for 6 min. In each field of interest, the fluorescence intensities of 10 cells were quantified using LSM Image Browser software

    Journal: Bone Research

    Article Title: Piezo1 channel activation in response to mechanobiological acoustic radiation force in osteoblastic cells

    doi: 10.1038/s41413-020-00124-y

    Figure Lengend Snippet: Fluorescence imaging of calcium oscillation and the effects of LIPUS stimulation on different groups of cells. a After LIPUS stimulation, the Fluo-8-labeled cells exhibited increased intracellular calcium levels at different time points. The red arrows show the two-cell calcium oscillation phenomenon. Scale bars, 50 μm. b Representative intracellular calcium traces of three groups of cells (MC3T3-E1, shRNA-Piezo1, and GsMTx4-treated cells) are shown as the fold increase in Fluo-8 intensity in response to LIPUS stimulation. The experiment was performed for six total minutes, including 1 min of baseline without LIPUS stimulation, 3 min of active stimulation (between the two red lines), and 2 min of regression. Time-lapse sequences were collected every 1.8 s for 6 min. In each field of interest, the fluorescence intensities of 10 cells were quantified using LSM Image Browser software

    Article Snippet: In the GsMTx4 inhibition experiments, 1.5 mL of α-MEM with GsMTx4 (TOCRIS, MN, USA) at a concentration of 4 μmol·L −1 was added to the dishes.

    Techniques: Fluorescence, Imaging, Labeling, shRNA, Software